In-Vivo Evidence: Behaviour & Analgesia in Rodents
Summary: In rats, speciogynine produced lower-lip retraction (a classic 5-HT₁A behaviour) and antinociception on the hot-plate test; both were blocked or attenuated by WAY-100635 (5-HT₁A antagonist), pointing to 5-HT₁A-linked mechanisms in vivo.
Key points:
Behavioural 5-HT₁A signature + antagonist reversal → mechanistic support for serotonergic mediation.
Caveat: dosing routes (i.p./i.v.) and first-pass metabolism complicate attribution to the parent vs 9-O-desmethyl metabolite.
